It is essential to uncover which chemicals fuel phage activity. Because phages attack and kill bacteria, if we understand how to manipulate them, they could work as powerful, natural antibiotics.
A recent study set out to expand the list of compounds that induce phage activity. The scientists from San Diego State University, CA, published their findings in the journal Gut Microbes. They hope that their results will introduce the “possibility of using diet to intentionally landscape the human gut microbiome via prophage induction.”
“We could actually tackle certain conditions by adjusting the foods we consume that will affect microbial diversity, which in turn will influence health and diseases.”
Research associate Lance Boling
To investigate, the researchers chose a wide range of compounds that might influence phage activity. They selected a range of bacteria from two phyla that are common in the gut: Bacteroidetes and Firmicutes. They included both beneficial and pathogenic strains of bacteria.
From 117 food compounds, they narrowed down their search to just 28. The researchers observed the growth of bacteria in the presence of each specific compound; they also observed its growth without the compound as a control. Next, they used flow cytometry, a process that is sensitive enough to detect unimaginably small virus particles.
Of the 28 candidates, 11 compounds produced levels of virus particles at a rate higher than the controls, which signifies that they influenced phage activity.
Some of the most significant phage boosts occurred in the presence of clove, propolis (a compound produced by bees), uva ursi (also known as kinnikinnick or bearberry), and aspartame.
The most potent prophage inducer was stevia, which is a plant-derived sugar substitute. With some species of the bacterial strains, stevia increased the number of virus particles by more than 400%.
Conversely, some foods reduced the number of virus particles; most notably, these included rhubarb, fernet (a type of Italian liquor), coffee, and oregano.
To complicate matters, some compounds boosted phage activity associated with some bacteria, but reduced phage activity related to others; these compounds include toothpaste, grapefruit seed extract, and pomegranate.
According to the authors, one of the most potent antibacterial foods was hot tabasco sauce, which “reduced the growth of all three [gastrointestinal] species, except the opportunistic pathogen P. aeruginosa, by an average of 92%.”
Tabasco contains vinegar, but when they tested vinegar alone, it only reduced bacterial growth by 71%. They believe that capsaicin — the spicy compound in chilis — may explain the additional antibacterial capabilities. However, in the experiments with tabasco, no virus particles were found, so phages are unlikely to be involved.
These findings are important. Scientists now know that the microbiome can influence our physical and mental health; it can also cause inflammation and increase cancer risk. If scientists can work out how to alter the microbiome in specific ways, they can, in theory, remove or reduce these risks.
As one of the authors, Forest Rohwer, explains, “The ability to kill specific bacteria, without affecting others, makes these compounds very interesting.”
The new list of compounds is by no means exhaustive, of course, as Rohwer says, “There are probably thousands of compounds that would be useful for eliminating unwanted bacteria.”
The authors hope that scientists will continue along these lines. They also explain that scientists will need to try to figure out the molecular mechanisms that switch the phage from inactivity to activity.